欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (15): 215-220.

所属专题： 生物技术； 园艺

• 农学 农业基础科学 • 上一篇    下一篇

桐花树抗重金属铅胁迫的谷胱甘肽及抗氧化酶作用

许丽璇 王春凤 王慧云 蔡建秀   

  • 收稿日期:2009-12-29 修回日期:2010-04-14 出版日期:2010-08-05 发布日期:2010-08-05

Effects of Lead Stress on Activities of Glutathione and Antioxidant Enzymes in Aegiceras Corniculatum Seedling

  • Received:2009-12-29 Revised:2010-04-14 Online:2010-08-05 Published:2010-08-05

PDF (PC)

947

摘要：

摘要：为揭示Pb2+对红树植物的环境毒性和植物的抗性与受害机制，以及为治理海洋河口重金属污染提供科学依据，通过在含铅（Pb2+）0.1-3 mmol/L的系列海水中水培桐花树幼苗，研究幼苗根系丙二醛、过氧化氢含量、POD、SOD值及根系和叶片谷胱甘肽过氧化物酶含量的变化情况。实验结果表明：在Pb2+胁迫下，桐花树根系MDA含量随Pb2+浓度的增加及胁迫时间的延长均有所提高。桐花树根系过氧化氢含量随着胁迫时间的延长而增加，但与Pb2+浓度的变化没有明显的规律，这主要是因为影响植物体内过氧化氢含量的因素较复杂。POD、SOD酶活性在Pb2+浓度小于1 mmol/L时升高，大于1 mmol/L时活性降低。桐花树根系谷胱甘肽过氧化物酶活性随胁迫时间的延长呈现出先升后降的趋势，叶片谷胱甘肽过氧化物酶活性随胁迫时间的延长而逐渐下降，而两者的谷胱甘肽过氧化物酶活性均随着胁迫浓度的增加而下降。

关键词: 棘胸蛙, 棘胸蛙, 生物技术, 繁殖, 养殖, 调控

Abstract:

Abstract: Abstract: In order to study is to reveal the influence of Pb2+ resulted in the environmental toxicity and the mechanisms of resistance and victims of mangrove plants, and provide a theoretical basis for the management of heavy metal pollution in marine estuaries. The aegiceras carniculatum seedlings were cultured in a series of seawater which contained 0.1-3 mmol/L Pb2+, Studying the changes of malondialdehyde, hydrogen peroxide，POD and SOD in the seedling roots and the changes of peroxidase in the seedling roots and leaves. The results of experiments showed that both improving the concentration of Pb2+ and extending stressing time increased the MDA contents in seedling roots. The hydrogen peroxide in seedling roots increased with the extending stressing time, but did not change with the strength of Pb2+. It was mainly because of the complex factors impacting the hydrogen peroxide in plant. The activity of POD and SOD increased when the strength of Pb2+ were less than 1mmol/L, and decreased when the strength greater than 1mmol/L With stressing time becoming longer, the activity of glutathione peroxidase in seedling roots had a trend of increasing first and then decreasing, while the activity of glutathione peroxidase in leaves decreasing. Both the activity of glutathione peroxidase in seedling roots and leaves decreased with the increasing strength of Pb2+.