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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (14): 8-12.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

三株鸭肝炎病毒1型全基因序列分析

施少华 施少华 陈红梅 万春和 程龙飞   

  • 收稿日期:2010-01-22 修回日期:2010-03-22 出版日期:2010-07-20 发布日期:2010-07-20
  • 基金资助:
    国家自然科学基金项目;现代农业技术体系建设专项资金

Genomic sequence analysis of three strains of duck hepatitis virus type 1

  • Received:2010-01-22 Revised:2010-03-22 Online:2010-07-20 Published:2010-07-20

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摘要： 【研究目的】为分析三株1型鸭肝炎病毒（DHV）的遗传变异和进化关系；【方法】采用RT-PCR和5'-/3'-RACE.方法测定三株1型鸭肝炎病毒全基因序列；【结果】研究结果表明，不含poly（A）尾巴的3株病毒的基因组全长为7691~7700 nt，基因组均仅含一个长度为6750 nt的ORF，625~627 nt 5'端非翻译区和325~328 nt 3'端非翻译区(UTR)。将3株病毒株与GeneBank公布的其他DHV-1全基因序列及小RNA病毒科的其它属代表病毒株进行序列分析表明，聚蛋白均被分割成为12个蛋白，其中VP1的变异性最大，180~187位为高变区。3株病毒与DHV-1参考毒株核苷酸序列同源性在93.7%~98.8%，氨基酸序列同源性在96.9%~98.8%，DHV-1各株在小RNA科病毒多聚蛋白氨基酸序列进化树上形成一个独立的进化分支；【结论】DHVs-1病毒间核苷酸和氨基酸仍较保守，它们在进化中可将其归为小RNA病毒科的一个新属。

关键词: SRAP, SRAP, 分子标记, 蔬菜, 应用

Abstract: [OBJECTIVE]This experiment was conducted to study the genetic variations and phylogenetic analysis of three strains of duck hepatitis virus type 1(DHV-1); [METHOD]The complete sequences of three DHVs-1(FZ86, FZ99 and FZ05) were acquired by RT-PCR and 5'-/3'-RACE; [RESULTS]The results showed that the full genomic length of three strains virus were 7691-7700 nt without poly(A) tail, including 625-627 nt 5’UTR, followed by a long open reading frame(ORF) of 6750 nt and 325-328nt 3’UTR. The nucleotide sequences analysis of three DHV-1 strains and the cited DHV-1 isolates showed that their polyproteins can be cleaved into 12 proteins. In the 12 polyproteins, the VP1 polyprotein had large variation of aa180-187. The homologies of three DHVs-1 and other DHVs-1 were within 93.7%-98.8%, while the amino acid homology were 96.9%-98.8%. The DHV-1 strains clustered together and formed a separated sublinge with other numbers of the family Picornaviridae; [CONCLUSION] DHVs-1 are conserved on nucleotide and amimo acid , and belong to a new genus in the family Picornaviridae .