欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (12): 130-134.

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

冬樱花愈伤组织诱导和抑制褐化初探

和凤美1,朱永平2,杨晓红3,李璇1,邵琬珊1   

  • 收稿日期:2010-02-01 修回日期:2010-02-28 出版日期:2010-06-20 发布日期:2010-06-20

The Primary Investigation on Callus Induction and Browning Prevention of Cerasus Cerassoides

He Fengmei1, Zhu Yongping2, Yang Xiaohong3, Li Xuan1, Shao Wanshan1   

  • Received:2010-02-01 Revised:2010-02-28 Online:2010-06-20 Published:2010-06-20

PDF (PC)

1111

摘要：

以冬樱花叶片、花托、花梗、子房和苞片为外植体，在附加不同浓度的生长素NAA和细胞分裂素6-BA组合的MS培养基上进行愈伤组织诱导并探讨愈伤组织褐化的抑制，实验结果表明：在供试的培养基中，叶片的愈伤组织诱导率最高，花器官愈伤组织诱导时，花梗诱导率最高，其次为花托、子房，而苞片诱导率为0。缩短外植体消毒时间、遮光及加入活性炭，可有效降低愈伤组织的褐化。

关键词: 原生质体, 原生质体, 制备, 应用

Abstract:

In this paper, we studied induction of callus and browning prevention on MS addition with different concentration of 6-BA and NAA using receptacle, peduncle, ovary and leaf blade of cerasus　cerassoides as explants. The results showed that the callus induction rate of leaf was the highest in all tested culture media and explants, and among callus induction of floral organogenesis, peduncle was the best, followed by receptacle and ovary, and that of bracteolate was the lowest.Browning of callus can be effectively decreased with shorting explants disinfection time, keeping away from light, addition with active carbon.