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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (14): 78-81.

所属专题： 园艺

• 生物技术科学 • 上一篇    下一篇

中国白菜小孢子离体培养和双单倍体植株再生

陈琳 刘克德 吴江生 孙秀丽 汪 频 石淑稳   

  • 收稿日期:2010-02-05 修回日期:2010-04-15 出版日期:2010-07-20 发布日期:2010-07-20
  • 基金资助:
    国家支撑计划航天育种项目

In vitro culture and plant regeneration of the microspores from Brassica campestris L. （ssp.chinensis and ssp.pekinensis)

  • Received:2010-02-05 Revised:2010-04-15 Online:2010-07-20 Published:2010-07-20

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摘要： 摘 要: 采用略加修改的NLN培养基（无机大量元素1/2）和甘蓝型油菜小孢子的胚状体诱导方法，培养了5个中国菜用小白菜品种和1个中国大白菜品种的小孢子，进行了双单倍体的诱导。结果表明，不同品种的小孢子产胚量差异很大，在接种的6个基因型中，有5个诱导出胚，诱导成功率83.33％ 。其中的中萁青抗热605产胚量最高，是8.676个胚／10蕾。这些胚在添加了NAA和PP333（多效唑）的B5培养基发育成健壮的小植株。关于6个基因型的小孢子胚胎发生能力，还有待进一步研究。

关键词: 动力学, 动力学, 吸附, 东北, 镉, 非线性拟合

Abstract: Abstract：The microspores of 5 genotypes of Brassica campestris L. ssp.chinensis and 1 genotype of Brassica campestris L. ssp.pekinensis were cultured in vitro using modified NLN medium (1 / 2 of inorganic major element) and the procedure of microspore culture of Brassica napus. The results showed that the embryo yield between genotypes was obviously different. The embryos regenerated from the microsores of 5 out of 6 genotypes with 83.33% of tested materials.The embryo yield of zhongqiqingkangre605 was the highest with 8.676 embryos per 10 buds.All embryos produced from the microspores of 5 genotypes developed into stronger plantlets on B5 medium added NAA and PP333. The embryogenesis capacity of microspores of 6 genotypes needs to be further studied.