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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (15): 168-171.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

麻栎的组织培养与快速繁殖

赵丹 诸葛强 唐罗忠   

  • 收稿日期:2010-02-17 修回日期:2010-03-15 出版日期:2010-08-05 发布日期:2010-08-05
  • 基金资助:

    国家公益性行业科研专项“麻栎炭用林定向培育及高效利用关键技术研究”;人力资源和社会保障部留学回国人员资助项目“麻栎能源林新品种选育及栽培技术研究”

Tissue Culture and Rapid Propagation of Quercus acutissima

  • Received:2010-02-17 Revised:2010-03-15 Online:2010-08-05 Published:2010-08-05

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摘要：

以麻栎幼苗茎段为外植体，以MS为基本培养基，研究了不同种类和不同浓度生长调节物质对麻栎组织培养的影响。结果表明，芽诱导最佳培养基为MS+6-BA1.0 mg?L-1，培育21天左右，腋芽可长至1～2 cm，展叶3～4片，发芽率达83.3%；芽增殖最佳培养基为MS+6-BA1.0 mg?L-1+NAA0.02 mg?L-1，增殖系数为4.6；最佳生根培养基为1/2MS+NAA0.5 mg?L-1，培养4周后生根率可达87%。

关键词: 微生态饲料添加剂, 微生态饲料添加剂, 研制, 肉牛, 生理生化指标, 影响

Abstract:

Effects of plant hormones to the tissue culture of Quercus acutissima were studied by means of seedling stems as explants and MS as basic medium. The results showed that the best medium for the bud induction was MS +6-BA1.0 mg?L-1. The bud was as long as 1 to 2 cm with 3 to 4 leaves after three weeks, and the germination rate was 83.3%. The best medium for the bud proliferation was MS +6-BA1.0 mg?L-1 + NAA0.02 mg?L-1, and the proliferation rate was 4.6. The best medium for rooting was 1/2MS + NAA0.5 mg?L-1, and the rooting rate was about 87% after four weeks.