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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (13): 324-327.

所属专题： 园艺

• 植物保护 农药 • 上一篇    下一篇

中药白鲜皮内生细菌TS-5生化特性及对番茄灰霉病菌的抑制作用

窦瑞木 雷清泉 曹克强   

  • 收稿日期:2010-03-17 修回日期:2010-03-24 出版日期:2010-07-05 发布日期:2010-07-05
  • 基金资助:

    河北省自然科学基金资助项目

Study on Physiological and Biochemical Characters of Endophytic Bacterial Strain TS-5 from Densefruit Pittany Root-bark and Its Antibacterial Activity to Botrytis cinerea

  • Received:2010-03-17 Revised:2010-03-24 Online:2010-07-05 Published:2010-07-05

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摘要：

为了探求中草药植物内生菌对植物病原菌的抑制作用，采用对峙试验、活体防效测定、革兰氏染色、芽孢染色以及生理生化特性、生长条件测定等方法，研究了从中药白鲜皮中获得的一株内生细菌TS-5对灰霉菌的抑制作用。试验结果表明，此内生细菌对灰霉菌有较强且稳定的抑制作用，在培养皿对峙试验中，抑菌带达10 mm，在活体上对病害的抑制率为33.72%；经形态学观察和生理生化反应，确定此菌株属枯草芽孢杆菌（Bacillus subtilis）；其最适生长温度为30℃，最适pH值为7，最佳培养时间为48 h，通气量越大菌株的生长情况越好。

关键词: 柑橘, 柑橘, 功能性成分, 提取, 检测

Abstract:

The endophytic bacterial strain TS-5 from medicinal plants of Densefruit pittany, root-bark was tested for its inhibiting effect against Botrytis cinerea. The physiological and biochemical characters, antagonistic activity, classification and growth condition of TS-5 in vitro were measured. The results showed that the strain had antagonistic activity against the gray mold disease, the inhibition zone was 10mm , antagonistic rate on leaves was 33.72%. According to the morphology observation and physiological and bio-chemical reactions, the strain was identified as Bacillus subtilis. Tests showed that the optimum growth condition is 30℃ at pH 7 for 48 hour. The bacterial strain is aerobic.