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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (19): 263-268.

• 植物保护 农药 • 上一篇    下一篇

不同寄主致病疫霉菌群体遗传结构的SSR比较分析

陈昌盛 陈庆河 李本金 翁启勇   

  • 收稿日期:2010-04-22 修回日期:2010-05-26 出版日期:2010-10-05 发布日期:2010-10-05
  • 基金资助:

    国家自然科学基金;国家自然科学基金

Comparison of Phytophthora infestans population genetic structure from different hosts based on SSR analysis

  • Received:2010-04-22 Revised:2010-05-26 Online:2010-10-05 Published:2010-10-05

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摘要：

用SSR分子标记技术对采集自中国不同地区不同寄主来源的60个致病疫霉菌(Phytophthora infestans)群体遗传多样性进行比较分析。结果显示，番茄致病疫霉菌群体Nei氏基因多样性指数、Shannon信息指数及有效等位基因数等遗传多样性指数(分别为0.1528、0.2592、1.2170)均高于马铃薯致病疫霉菌（分别为0.1395、0.2447、1.1913），表明番茄致病疫霉菌群体遗传多样性高于马铃薯致病疫霉菌群体；聚类分析结果表明：SSR标记分析的遗传系谱与菌株对甲霜灵敏感性、交配型无相关性，而与MtDNA单倍型有一定的相关性。

关键词: 甜菜M14品系, 甜菜M14品系, 褐斑病抗性, 酶活性, 氨基酸含量

Abstract:

SSR molecular markers were assayed to determine the population genetic diversity of 60 representative Phytophthora infestans isolates from China. The results showed that the genetic diversity index of Nei’s genetic diversity index,Shannon information index and the effective number of alleles（0.1528、0.2592、1.2170）of host plant tomato pathogenic Phytophthora species were higher than those (0.1395、0.2447、1.1913) of the host plant potato Phytophthora species,the results indicated the genetic diversity of host plant tomato Phytophthora species was higher than the host plant potato Phytophthora species.The results also showed there is not relationship between the genetic lineage and metalaxy sensitivity and Mating type, but there is relationship between the genetic lineage and mtDNA Haplotype.