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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (24): 173-178.

• 农学 农业基础科学 • 上一篇    下一篇

PEG6000渗透胁迫下应用电导法对披碱草属种质幼苗抗旱性初步研究

德英 赵来喜 穆怀彬   

  • 收稿日期:2010-05-14 修回日期:2010-07-30 出版日期:2010-12-20 发布日期:2010-12-20

Conductance Method Determination to the Drought Resistance of Elymus Species under PEG6000 Stress

  • Received:2010-05-14 Revised:2010-07-30 Online:2010-12-20 Published:2010-12-20

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摘要：

以15%聚乙二醇（PEG6000）作渗透介质，应用电导法对59份7种披碱草属（Elymus L.）种质幼苗进行了抗旱性初步研究。结果表明，59份种质材料按照评价标准可分为4级，抗旱性可分为强、较强、中等、弱。抗旱性强的种质5份，占供试种质的8.48%,包括PJ001、PJ003、PJ005、PJ006、PJ027；抗旱性较强的种质16 份，占供试种质的27.12%，包括PJ002、PJ004、PJ007、PJ009、PJ010、PJ011、PJ012、PJ013、PJ014、PJ017、PJ018、PJ021、PJ036、PJ042、PJ073、PJ093；抗旱性中等的种质20份，占供试种质的33.90%，包括PJ025、PJ029、PJ033、PJ034、PJ035、PJ038、PJ039、PJ054、PJ066、PJ067、PJ068、PJ090、PJ097、PJ098、PJ099、PJ100、PJ108、PJ109、PJ113、PJ114；抗旱性弱的种质18份，占供试种质30.50%，包括PJ019、PJ022、PJ037、PJ069、PJ070、PJ074、PJ075、PJ077、PJ078、PJ092、PJ101、PJ105、PJ107、PJ110、PJ111、PJ112、PJ115、PJ116。电导法测定抗旱性具有相当程度的灵敏度和精确性，是快速筛选抗旱性种质的有效手段。

关键词: 长白猪, 长白猪, 甘露聚糖结合凝集素C, 基因克隆, 序列分析

Abstract:

By 15% polyethylene glycol (PEG6000) for stress medium, artificial simulation drought condition, to evaluate anti-drought of 59 germplasm materials which include 7 Elymus species. The results showed, 59 germplasm materials can be divided into four levels according to the evaluation standards, which are very high, high, medium, low. Germplasm materials of very high drought resistance have five, for 8.48% of the whole materials, including PJ001, PJ003, PJ005, PJ006, PJ027. High drought resistance have 16, for 27.12% which are PJ002, PJ004, PJ007, PJ009, PJ010, PJ011, PJ012, PJ013, PJ014, PJ017, PJ018, PJ021, PJ036, PJ042, PJ07, PJ093. Medium have 20, for 33.9%, which are PJ025, PJ029, PJ033, PJ034, PJ035, PJ038, PJ039, PJ054, PJ066, PJ067, PJ068, PJ090, PJ097, PJ098, PJ099, PJ100, PJ108, PJ109, PJ113, PJ114. And low drought resistance have 18, for 30.50%, which are PJ019, PJ022, PJ037, PJ069, PJ070, PJ074, PJ075, PJ077, PJ078, PJ092, PJ101, PJ105, PJ107, PJ110, PJ111, PJ112, PJ115, PJ116. Conductance method has considerable sensitivity and accuracy, is the effective means of screening drought resistance germplasm rapidly.