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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (2): 119-122.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

脐橙离区β-甘露聚糖酶基因片段克隆和序列分析

任艳芳 刘厚宇 何俊瑜   

  • 收稿日期:2010-06-13 修回日期:2010-08-10 出版日期:2011-01-14 发布日期:2011-01-14
  • 基金资助:

    果柄离区甘露聚糖酶基因的克隆及其在脱落过程中的表达

Cloning and Sequence Analysis of Endo-β-mannanase Gene Fragment from Abscission Zone of Navel Orange

  • Received:2010-06-13 Revised:2010-08-10 Online:2011-01-14 Published:2011-01-14

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摘要：

β-甘露聚糖酶是一种参与细胞壁半纤维素组分甘露聚糖降解的关键酶。为探究该酶与器官脱落的关系，以纽荷尔脐橙（Citrus sinensis Osbeck）果柄离区为材料，采用同源PCR克隆策略，扩增脐橙β-甘露聚糖酶基因片段。结果表明：该序列长度274 bp，编码了一段由91个氨基酸残基组成的β-甘露聚糖酶保守区片段。同源比对表明，该序列与GenBank上其他已有的植物β-甘露聚糖酶氨基酸序列的同源性在53%~65%，推断克隆得到的片段是脐橙β-甘露聚糖酶的cDNA片段。

关键词: 白腐菌, 白腐菌, 萘酚绿-B, 脱色, 优化

Abstract:

Endo-β-mannanase is an important enzyme involved in the mobilization of mannan, which is the component of hemicellulose. In order to investigate the relationship between endo-β-mannanase and organ abscission, the PCR based homologous cloning strategy was used to identify endo-β-mannanase (CiMan) genes from the fruit stalks abscission zone of navel orange (Citrus sinensis Osbeck). The results showed that a 274 bp cDNA encoding 91 amino acids was successfully cloned and characterized．Multiple alignment analysis of amino acid sequences of the putative CiMan cDNA fragment was 53%-65% homologous with other sequences from Genbank. It was supposed that the cDNA fragment was the endo-β-mannanase of navel orange.