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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (1): 149-153.

• 农学 农业基础科学 • 上一篇    下一篇

IP3敏感的钙离子通透性通道参与茉莉酸诱导的钙动员

李洋洋 杜希华 于涌鲲 赵福宽 孙清鹏   

  • 收稿日期:2010-06-22 修回日期:2010-08-05 出版日期:2011-01-07 发布日期:2011-01-07
  • 基金资助:

    国家自然科学基金;国家自然科学基金;北京市自然科学基金;北京市科技新星计划

IP3 Sensitive Calcium Channel Involved in the Jasmonic Acid Induced Calcium Mobilization

  • Received:2010-06-22 Revised:2010-08-05 Online:2011-01-07 Published:2011-01-07

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摘要：

以低温导入法将钙离子荧光探针Fluo-3/AM导入拟南芥叶片细胞中，利用LAS AF（Leica Application Suite-Advanced Fluorescence）软件记录肝素对茉莉酸（JA）诱导的胞内钙离子荧光强度的变化。结果显示，经不同浓度的肝素预处理后，拟南芥叶细胞中胞内钙离子的荧光强度降低，再用100 μmol/L JA处理时，其荧光强度升高，但仅与未经肝素处理的荧光强度相当。实验证明，肝素预处理可抑制JA诱导的胞内钙离子浓度的升高。

关键词: 杜仲叶, 杜仲叶, 绿原酸, 生物活性, 提取纯化, 应用

Abstract:

Arabidopsis thaliana leaves were labeled by fluorescent probe Fluo-3/AM under low temperature at 4 ℃ to measure the fluorescent intensity of intracellular Ca2+ which was pretreated with heparin on jasmonic acid (JA)-induced. The results showed that the fluorescent intensity of [Ca2+]cyt was reduced after pretreated with different concentration of heparin, and then treated with 100 μmol/L JA, the fluorescent intensity of [Ca2+]cyt was close to the fluorescent intensity which was not pretreated with heparin. The experiment showed that the pretreatment with heparin could inhibit the increase of the intracellular Ca2+ concentration significantly which JA-induced in leaves of Arabidopsis thaliana.