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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (3): 143-146.

所属专题： 烟草种植与生产

• 农学 农业基础科学 • 上一篇    下一篇

体视显微镜法观察烤烟花芽分化的研究

段玉琪 晋艳 杨宇虹 金 磊 周冀衡 黄成江   

  • 收稿日期:2010-07-14 修回日期:2010-09-26 出版日期:2011-02-05 发布日期:2011-02-05
  • 基金资助:

    烤烟早花发生条件及预防技术研究

Study on the Floral Differentiation of Flue-cured Tobacco by Stereomicroscope

  • Received:2010-07-14 Revised:2010-09-26 Online:2011-02-05 Published:2011-02-05

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摘要：

对低温诱导后的烤烟茎端分生组织花芽分化过程进行了体视显微镜法观察，镜检结果表明，该方法比扫描电镜法和石蜡切片法具有明显的简便性和快捷性。此方法对茎端部位的空间特征的凸显性优于扫描电镜的方法，具有较好的整体性；同时与扫描电镜法观察到的烤烟侧枝花序分化相一致，这是石蜡切片法难以捕捉和发现的。

关键词: 棉花, 棉花, 纤维品质, 气象因子

Abstract:

The floral bud differentiation processes on apical meristem of flue-cured tobacco induced with low temperature were observed by stereomicroscope. The results showed that this method was simpler and faster than that of scanning electron microscope and paraffin section. It revealed that the stereomicroscope is more superior in showing the spatial characteristics of meristem than that of the scanning electronic microscope method, and the image is more complete. It also validated the uniqueness of the inflorescence initiation of flue-cured tobacco lateral shoot which was observed by scanning electron microscope, and this uniqueness could not be captured or found by paraffin section.

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