欢迎访问《中国农学通报》,

中国农学通报 ›› 2010, Vol. 26 ›› Issue (23): 37-41.

所属专题: 水稻

• 生物技术科学 • 上一篇    下一篇

控制水稻RIL群体化感作用的QTL定位研究

贾小丽 林文雄   

  • 收稿日期:2010-08-16 修回日期:2010-09-15 出版日期:2010-12-05 发布日期:2010-12-05
  • 基金资助:

    核桃青皮杀螨活性成分及作用机理研究

Qtl analysis on allelopathyof the recombinant inbred lines(RILs) in rice( Oryza sativa L. )

  • Received:2010-08-16 Revised:2010-09-15 Online:2010-12-05 Published:2010-12-05

摘要:

此研究以“莱蒙”(弱化感)和“多拉”(强化感)水稻杂交产生的重组自交系(recombinant inbred lines,RILs)及其亲本为供体植物,并以稻田主要杂草稗草为受体植物,采用迟播共培法测定与各家系及亲本共培稗草的根长,并转化成抑制率来表征化感作用。结果表明群体及亲本根长抑制率基本呈正态分布,且用它来表征化感作用是适合的。运用分子标记技术构建了该群体的遗传图谱,共97个分子标记,覆盖水稻12条连锁群。QTL定位检测到控制水稻化感作用的5个QTL,分别位于1、1、5、9、9号连锁群,解释了23.97%、15.21%、13.89%、18.63%、6.64%的遗传变异。进一步证实水稻化感作用存在主效QTL,同时为分子育种提供依据。

关键词: 烤烟, 烤烟, 植烟海拔, 卷烟烟气

Abstract:

In this paper, we used the recombinant inbred lines (RILs) drived from the crosses by the rice cultivar Laymen (weak allelopathy) with Dollar (strong allelopathy) as genetic material. Allelopathic effect of RIL population and their parents were detected by relay-seeding co-culture in agar(RCA). The results indicated that root-length inhibitory effects of Barnyardgrass by the RIL population was close to normal distribution, and used as allelopathic index was for genetic analysis. With the aid of the molecule marker technology, we got the genetic map of the lines, covering with all the chromosomes and having ninety-seven molecule markers. Five QTLs associated with allelopathy against the target weed were detected and located in 1, 1, 5, 9, 9 linkage groups, and explained 23.97%, 15.21%, 13.89%, 18.63%, 6.64% of genetic variation respectively. Further confirmed the existence of rice allelopathy main effect QTL ,also provide the basis for the molecular breeding.

中图分类号: