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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (9): 148-151.

• 农学 农业基础科学 • 上一篇    下一篇

黑农51子叶节和胚尖再生体系的建立及优化

张艳 满为群 南相日 李柱刚   

  • 收稿日期:2010-09-03 修回日期:2010-10-11 出版日期:2011-04-25 发布日期:2011-04-25
  • 基金资助:

    国家转基因重大专项

Stablishment and Improvment on Regeneration Systems of Heinong51 Cotyledonary Nodes and Embryonic Tips

  • Received:2010-09-03 Revised:2010-10-11 Online:2011-04-25 Published:2011-04-25

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摘要：

为建立一个高效的大豆再生体系用于大豆的遗传转化，选用黑农51的子叶节和胚尖作为外植体，建立了黑农51的子叶节和胚尖再生体系，并研究了6-BA和IBA对大豆再生的影响。结果表明，黑农51子叶节最适芽诱导培养基为MSB5+1.0 mg/L 6-BA+ 0.2 mg/L IBA，最适生根培养基为MSB5+1.0 mg/L IBA；胚尖最适芽诱导培养基为MSB5+0.4 mg/L 6-BA+0.2 mg/L IBA，最适生根培养基为MSB5+2.0 mg/L IBA。黑农51子叶节再生体系的出芽率、出芽数、芽伸长数和生根率四个指标均高于胚尖再生体系，更适合作为遗传转化的受体材料。

关键词: 番红花, 番红花, 球茎, 激素

Abstract:

To establish a high frequency regeneration system for the gene transformation of soybean [Glycine max(L.) Merr.], cotyledonary nodes and embryonic tips from Heinong51 were used as the explants. Regeneration systems of cotyledon nodes and embryonic tips from Heinong51 were established in this research. Effect of 6-benzylaminopurine (6-BA) and indolebutyric acid (IBA) on soybean regeneration was studied. The results indicated that the optimal shooting induction medium of cotyledonary node from Heinong51 was MSB5 added 1.0 mg/L 6-BA and 0.2 mg/L IBA and the optimal rooting medium of cotyledonary node was MSB5 added and 1.0 mg/L IBA. The optimal shooting induction medium of embryonic tip from Heinong51 was MSB5 added 0.4 mg/L 6-BA and 0.2 mg/L IBA and the optimal rooting medium of embryonic tip was MSB5 added and 2.0 mg/L IBA. In comparison with regeneration system of embryonic tips, Heinong51 regeneration system of cotyledon nodes was superior in shoot regeneration frequency , shoot regeneration number and shoot elongation number. The cotyledon nodes from Heinong51 was more suitable for receptor materials in soybeans genetical transformation.