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Chinese Agricultural Science Bulletin ›› 2008, Vol. 24 ›› Issue (11): 44-47.doi: 10.11924/j.issn.1000-6850.2008-0693

Special Issue: 生物技术； 畜牧兽医

• 畜牧兽医科学 • Previous Articles     Next Articles

Effects of SU-MIAN-XIN on CYP3A1 and CYP2E1 in Rat Primary Cultured Hepatocytes

Xu Lina, Du Jinliang, Ma Yuzhong   

  • Received:2008-08-15 Revised:2008-09-09 Online:2008-11-05 Published:2008-11-05

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Abstract: Rat primary cultured hepatocytes were isolated by two-step collagenase digestion method and treated with gradient concentration of SU-MIAN-XIN and BAO-DING-NING, and the expression of cytochrome P450 3A1 (CYP3A1) and cytochrome P450 2E1 (CYP 2E1) were determined by Western blot. The results as follows: 2-4×108 hepatocytes per rat were obtained and the viability was about 95%. The expression of CYP3A1 and CYP 2E1 in rat hepatocytes increased gradually with treatment of SU-MIAN-XIN and BAO-DING-NING. Rat primary cultured hepatocytes can be used for molecular mechanism of SU-MIAN-XIN metabolism, it provides a foundation for its clinical application.