Abstract: Root growth, shoots and roots biomass of spring maize(Nongda 364, Xianyu 335) were investigated with a pot experiment simulating low temperature stress. The experiment results showed that low temperature restrained root surface, root volume and length of axile root. There was significant genotypic variation in total root length and lateral root length in response to low temperature stress. The result showed that low temperature stimulated lateral root growth of Nongda 364, but inhibited lateral root growth of Xianyu 335. Analysis of biomass indicated that low temperature stress increased root/shoot.