Abstract:

As a key enzyme of citrate metabolism, citrate synthase shows a close relationship with the accumulation of citrate. In order to investigate the expression mode of citrate synthase gene, the cDNA encoding citrate synthase was cloned from Hamlin leaf by PCR. It was 1535 bp long and encoded a protein with 471 amino acids. Alignment analysis showed that citrate synthase gene had high homology with other plants. The samples were collected in four different periods after flowering. The expression of citrate synthase gene had no obvious change in flesh and peel of different periods, while was decreased during the development which had an identitcal tendency with the change of the amount of citrate.