Abstract:

To investigate the treatment mechanism of the paeonol (Pae) on the listeriosis and the secretion of NO and ET-1 in rat intestinal mucosa microvascular endothelial cells (RIMVECs) induced by listeriolysin O (LLO). RIMVECs were in culture divided into LLO+Pae, LLO, Pae and blank control group. Cells were assessed proliferation by MTT assay. The Changes of NO and ET-1 level were measured by the method of nitratase, ELISA and hybridization in situ. Compared with other groups, proliferation of LLO was very significantly decreased. The production of NO and ET-1 were enhanced for 12h, and NO/ET-1 ratio was lower. Pae could regulate imbalance of NO/ET-1 induced by LLO and improve the local microcirculation of intestine.