Abstract:

In this paper, in order to increase the enzyme activity of cellulase, using a solid state fermentation method to study the composition changes of corn straw after pretreatment. The solid to liquid ratio, temperature, pH and other effects on the enzyme production were also be investigated. The results showed that, the optimum conditions for cellulase were: the solid liquid ratio was between 1:(1.5-2), the same ratio of corn straw and wheat bran were used with 1.5% ammonium sulfate and natural pH for fermentation. The medium were inoculated with bacterial culture (20% v). In these conditions, the corn stalks after fermentation, enzyme activity was 300 U/g. All the results provided a theoretical basis for further study.