Abstract:

In order to induce low-temperature-resistant mutants of tomato with chemical mutagen, callus of totamo variety ‘TTI1103B-2’ were treated with different concentrations of ethyl methane sulphonate (EMS) and sodium azide (NaN3) for different time to determine the Semi-lethal dose and the appropriate processing time by the callus survival rate. The results showed that the survival rate of callus decreased with treated time at the same chemical mutagenesis concentration, meanwhile, the survival rate of callus dropped down by the chemical mutagenesis concentration at the same time treatment. Semi-lethal dose of EMS was 0.2% concentration for 20-40 min, and the concentration of NaN3 was 3.0 mmol/L for 40-60 min.