Abstract:

In order to solve the problem of Capsicum hybrid rapid identification of the problem, it is necessary to establish the hot pepper hybrid seed purity identification system indoor, 96 (Xingshu seeds 32 varieties of male, female and F1) as the test material, using 150 pairs of SSR primers and 96 materials were amplified by PCR, looking for the amplification of discrepancy. The amplification results found in 51 on 150 pairs of primers can amplify clear bands, 19 pairs of polymorphism was better, 5 pairs of primers can distinguish Xingshu seed 32 varieties of male, female and their F1. SSR markers can be quickly and accurately identify the purity to pepper hybrids were consistent with field identification, and low cost.