Abstract:

This study aimed to confirm the imported 9 serotypes of AHSV and have some explore and discovery on the differentiating method for AHSV. In the current study specific primers for differentiating each of the nine serotypes of AHSV were designed based on the L2 genome segment according to the reported references. RT-PCR method and sequencing were used. The RNA samples extracted from inactivated vaccine virus of the above 9 serotypes all could produce specific products amplified with the specific primers through RT-PCR method, and the amplification conditions were modified and optimized. The products amplified from the AHSV RNA had been sequenced and blasted through the internet. Conclusively, the developed RT-PCR method in this study is able to be employed as a technique for differentiating the 9 serotypes of AHSV, and we also confirmed the imported 9 serotypes of RNA.