Abstract:

In this study a RT-PCR ELISA method was established using the primer which was designed according to M gene sequence of AIV and labeled by biotin and DIG.. The optimal reaction conditions were determined including the streptavidin was 2?g/mL and anti-digoxigenin-POD dilution titer was 1:3000. This method had a strong specificity and high sensitivity. The sensitivity was more than 100 times compared to RT-PCR, so that it could overcome the difficulty for the little AIV in the samples．It provides a new way for the molecular epidemiologic investigation and early diagnosis of AIV