Abstract:

In order to study the molecular characteristics of the avian influenza virus (H9 subtypes) HN31 strain’s NS gene, its nucleotides were cloned into pMD19-T Vector by RT-PCR. The ligation product was transformed into the competent E.coli DH5α. The white colony was selected, and identified by PCR and EcoRⅠrestriction enzyme digestion. The positive plasmid was sequenced. The results show that the RT-PCR product is 919bp, HN31 strain’s NS gene length is 890bp. Through the phylogenetic analysis, the kinship with H6, H9 subtype is closer than with H5, H7, H3, H1 subtype of influenza virus.