Abstract:

In order to identify the differential expressed proteins between the small rubber particles (SRPs) and the large rubber particles (LRPs) in the latex of Hevea brasiliensis (rubber tree) and to unravel the molecular mechanisms of rubber biosynthesis, SRPs, LRPs and the total rubber particles were completely separated and purified from the fresh latex using differential centrifugation, and further detected through scanning electron microscope (SEM), respectively. One-dimensional SDS-PAGE analysis demonstrated that the protein profile of the LRPs was different with that of the SRPs, including the differentially expressed proteins with appropriate mass weight of 22.0 kDa, 24.5 kDa and 120 kDa, and so on. One protein expressed only on the SRPs, SRPP, was further identified using nano-LC MS/MS.The separation of LRPs and SRPs from the latex would facilitate both the identification of the differential expressed proteins between LRPs and SRPs, and the further investigation into the molecular mechanisms not only promote the biogenesis and the development, but also promote the rubber biosynthesis of the rubber particle of rubber trees.