Abstract:

In order to provide a theoretical basis for the occurrence, epidemic and control technologies of the disease, the variation and genetic structure of Colletotrichum gloeosporioides were explored. Sequence related amplified polymorphism (SRAP) analysis was used to detect the genetic variation in populations of the 25 C. gloeosporioides in different areas. Among 100 combinations of primer pairs screened, 6 primer pairs that amplified consistent polymorphic bands with DNA of 4 isolates were generated. They were further applied to amplify the genomic DNA of the 25 tested isolates. A total of 164 discernible bands were obtained, 156 of which showed polymorphism, and similarity coefficient was 95.12%. The dendrogram was constructed with the software NTSYSpc Version2.1 and the similarity coefficients were ranged from 0.61-0.95. The result indicated there was a close genetic relationship and abundant genetic diversity. Nonetheless, rich genetic variation existed among the tested isolates and there was no correlation between SRAP group and the geographic origin.