Abstract:

The aim of this study was to research milk protein gene expression—αs1-casein, β-casein, κ-casein of lactating bovine mammary epithelial cells between different generations; Using the real-time PCR method to detect milk protein gene expression of lactating bovine mammary tissue, the cells of primary generation (P0) derived from the bovine mammary tissue, the first generation (P1), the second generation (P2), the third generation (P3), the fourth generation (P4) and the fifth generation (P5); The results showed that expression of three target-genes was at different levels between the cells of primary generation and passages. The expression in the primary cells was significantly higher than P1-P5 cells (P<0.05), whereas P1-P5 cells had no significant difference (P>0.05). Moreover, from P0 to P5, the expression of αs1-casein, β-casein, κ-casein is gradually decreasing. So, P2-P5 BMECs could be used as a trial material.