Abstract:

SSR is a kind of PCR based molecular marker with high genetic polymorphism, codominant inheritance, rich content and easy-detection. Maize SSR technology is widely used in varieties identification, heterosis groups establishment, and so on. DNA extraction, PCR amplification and product testing are the key links of maize SSR molecular marker technology process. Despite of the appearance of some instruments that can integrate some links or even the entire process of the SSR operation procedure, the high price of them and expensive consumables cost inhibit their application .Therefore, in recent years, a lot of optimization and improvement have been put into effect to make the procedure more simplified and efficient. In this paper, three basic circles of maize SSR molecular marker technology are summarized, including DNA extraction, PCR amplification, product testing. The tendency of the experimental reagent integration, experiment manipulation standardization, the experimental process automation is shown according to analysis.