Abstract:

The aim was to isolate and identify a wild fruiting bogy-forming fungus which was obtained from Beijing Xiang-mountain. The mycelium was isolated from wild fruiting body-forming fungus. Genomic DNA was extracted from mycelium and fruiting body then used as template for the ITS gene PCR amplification. ITS gene fragments were purified and sequenced. The sequences were analyzed using phylogenetic method. PCR analysis showed that the isolated mycelium came from the wild fungus’s fruiting body. ITS sequencing and phylogenetic analysis indicated that the fungi belonged to the genus Agaricus. Genbank accession number of the ITS gene was JN907016. Phylogenetic analysis using MEGA4.1 software showed that the wild fungus had a high genetic distance, which was up to 97.2%, with A. fuscofibrillosus WC913. Identification of the isolated wild fungus using molecular biology method could analyze the genetic status and lay the foundation for the exploitation of the wild fungi.