Abstract:

In this study, maize (Zea mays) seedlings with three leaves were used as experimental materials, a NAC transcription factor gene fragment was cloned and sequenced. The specific primer was designed according to the specific conserved domain of NAC transcription factor, and a gene fragment was amplified by RT-PCR. The results showed that, the fragment was 230 bp, and belonged to the NAC family NAM subfamily. The fragment laid the foundation for cloning the full-length of maize NAC transcription and stress-resistant breeding in future.