Abstract:

The aim was to study the transcription regulation mechanism of MdGA3ox1 gene by studying the promoter region of MdGA3ox1 gene. The upstream sequence of ‘Fuji’ (Malus x domestica Borkh. cv. Fuji) MdGA3ox1 was attained by Genome Walking binding anchored PCR. The functional elements were analyzed by PLANTCARE. The sequence was inserted into the vector and transformed into Agrobacterium to verify the function preliminarily. The DNA sequence of 754 bp was amplified. The MdGA3ox1 gene promoter contained the basic elements: TATA-box, CAAT-box and other induced elements: light, gibberellin, enthylene and abscisic acid responsive elements. In order to verify the function of the sequence, the DNA fragments was inserted into the binary vector pCAMBIA1381 and named p1381-GA3ox1p. The plasmid of p1381-GA3ox1p was transformed into Agrobacterium EHA105. The analysis of GUS staining showed the promoter could drive the expression of GUS in transgenic Agrobacterium. The upstream sequence of MdGA3ox1 which contained the cis-elements with GA regulation was obtained. It also confirmed that the sequence had the promoter function.