Abstract:

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of infectious bronchitis virus(IBV); According to the sequences of IBV in GenBank, six primers were designed, and the reaction conditions were optimized. The results showed that the detection limit of this RT-LAMP method was 10 fg, whereas that of RT-PCR was 1 pg, suggesting the LAMP method was much more sensitive than the RT-PCR methods for IBV detection, the LAMP method for IBV detection was more convenient, rapid, specific and sensitive than the RT-PCR, the assay was performed in water bath within 1 h. Therefore, it was very suitable for detection in the field condition that would provide a novel rapid detection approach for IBV infection.