Abstract:

The aim of this article was to investigate the immunogenicity of rabbit haemorrhagic disease virus (RHDV) VP60 gene which was expressed in a prokaryotic system. The capsid protein VP60 gene of RHDV was cloned by RT-PCR and the product after purification was linked into PMD-18T vector. The plasmid was sequenced after extraction. Then the amply product which was digested by dual-enzyme was linked into prokaryotic expression vector PET28b and constructed prokaryotic expression vector. The recombinant plasmid was transformed into host strain E. coli RosettaTM induced by IPTG after the identification was right. The results showed that the expressed band of 62.0 KD was identified by SDS-PAGE and western blot. The purified recombinant protein reacted with the purified RHDV in ELISA. The prokaryotically expressed protein of RHDV VP60 showed well immunogenicity.