Abstract:

For the purpose of saccharification of dairy manure. Screening of several strains to construct strains of bacteria and to study its enzymatic mixed conditions for enzyme production.The results showed that the cellulose degradation rate of the F3-F9 pair accounted for 44.02%, which increased by 12.47%. Such as temperature, cultivation time, initial pH, S/L of medium, and addition of bran as well as nitrogen source etc had significant impact on cellulase productivity. compared with single strain fermentation. 0.1% urea was added to the medium, S/L was 1:2 and the inoculum concentration was 5%, the initial pH was 7, 40% bran were added to the medium and 24℃, FPase and CMCase of F3-F9 were 114.25 U and 1617.92 U respectively.