Abstract:

The aim was to study the genetic diversity and phylogenetic relationships of phytophthora capsici. in Hunan Province. The infected plants were collected from Zhijiang and Ningxiang. By separating and inoculating the detached leaves, two strains of phytophthora capsici were obtained. Their genes were amplified and sequenced by PCR with ITS and β-tubulin primers. The results and some known related sequences were used to construct a phylogenetic tree. The results showed that the disease could be identified by detached leaves inoculation, the length of ITS sequence was 770 bp and β-tubulin was 780 bp. Phylogenetic tree proved that the affinity of different phytophthora capsici variety might relate to their geographic origin, and also provided a basis for analyzing the genetic diversity of phytophthora capsici from Hunan Province.