Abstract:

The present study was designed to investigate the effect of hypertonic solution on the morphology, size, and dynamic of Mycoplasma hyopneumoniae (Mhp). With a final concentration of 1.8% NaCl, Mhp was stimulated for 1 min, 2 min, 4 min, 6 min, 8 min. Then these samples were analyzed by dyeing microscopic examination, particle size analyzer, CCU determination and PCR, respectively. The results showed that the morphology and size of Mhp had changed, such as shrinkage and round, smaller diameter, number of bacteria decreases rapidly, or even disappear, after which Mhp was effected for 2 min. CCU was reduced, and observation time was extended. The target band could be amplified for samples of Mhp. Therefore, it was concluded that the morphology and size had changed when Mhp were stimulated with a final concentration of 1.8% NaCl for 1~6 min, but they also have activity. Moreover, this study would also provided reference data for studing a new immunization route of Swine Mycoplasma Pneumonia vaccine and the blood circulation for Mhp by intramuscular injection.