Abstract:

To find out the effect of inoculation amount and C/N on anaerobic fermentation of the diary manure, the variations of productions of biogases, content of CH4 and H2S during the anaerobic fermentation of diary manure were studied under the inoculation amount of 10% and 20%，and C/N of 25:1, 27:1, 29:1 by regulated with vinasse respectively. The results showed that in the inoculation amount of 10% to 20%, start-up rate, biogas yield, methane gas yield and hydrogen sulfide gas yield increased with inoculation amount increasing, the peak time of biogas production was the same as methane production in different inoculation amount, of about the tenth day. The highest biogas yield and methane gas yield was in the C/N of 27:1, next was in C/N of 29:1 and C/N of 25:1, which illustrated that anaerobic fermentation performance has been promoted by higher C/N with vinasse. The variation of average content level of CH4 was the same as that of H2S, average content level of H2S was high when that of CH4 was high. To sum up, in this experiment the optimal conditions for anaerobic fermentation of diary manure were inoculation amount of 20% and C/N of 25:1, with the biogas yield and methane gas yield of 44.3 L/kg and 24.09 L/kg.