Abstract:

In order to find methods to promote Valsa ceratosperma to produce enough conidia in short time, the effects of six kinds of culture medium were tested on promoting V. ceratosperma to produce conidia. The results showed that V. ceratosperma produced a large number of conidia in barley, which was with shell and soaked in solution of honey and peptone. After inoculated to the medium of barley and cultivated for about 15 days in the darkness, the fungi produced a large number of pycnidia. The pycnidia extruded conidia threads after cultivated under UV light for about 25 days. About 11 pycnidia produced on each barley grain on average and 15% of the pycnidia released conidia. The conidia produced by on 70 g barley would make 1500~2000 mL conidia suspension which contained about 106 spores per milliliter. However, the quantity of conidia produced by different isolations promoted with the same method was different significantly.