Abstract:

In order to find methods for total DNA extraction from Malus herbarium specimens stored for many years and enriching polysaccharides and polyphenolics, modified CTAB methods were studied based on the conventional methods. The DNA templates were used directly in amplifying the whole nrITS region and cpDNA matK gene by adding precooling CTAB free buffer after treated without liquid nitrogen and improving the concentration of NaCl when precipitating. Utraviolet spectrophotometer and agarose gel electrophoresis methods were used to test the DNA quality. The results showed that modified CTAB methods were optimal to produce high quality and yields DNA and amplifiable in the polymerase chain reaction (PCR). This methods don’t need to grind with liquid nitrogen,saving manpower and reducing cost.In addition,adding CTAB free buffer and improving NaCl when precipitating were effective for the removement of polyphenolics and polysaccharides. The researches indicated that the modified CTAB was suitable for extracting genomic DNA from Malus herbarium leaves.