Abstract:

In order to meet the needs of the Chongqing pepper efficient breeding and germplasm innovation, pepper anther culture research was studied. Two processing pepper varieties 578 and 186 and a sweet pepper variety ‘Haifeng 28’ were selected as the experimental materials. The time and temperature of the pretreatment and pre-incubation were studied. By the orthogonal experimental design we explored the effects of the basic medium, plant growth regulating substances, activated carbon, silver nitrate, carbon source on anther culture. The results indicated that 35℃ for 7 days was the suitable pretreatment condition for all the three materials, the plant induction rates of pepper 578,186, sweet pepper ‘Haifeng 28’ were 4.86%, 2.50%, 0.56%. Two genotype-specific optimization of culture medium were established by orthogonal test analysis and one culture medium suited to the two genotypes was obtained. The ploidy identification showed that haploid plantlets with 12 chromosomes were regenerated from anthers of all the three genotypes. Socking the growing point with the mixture of 0.2% colchicine and saturated p-dichlorobenezene for 24 hours, double haploid plants were successfully obtained.