Abstract:

The aim was to study the action of genes encoding auxin signaling components in plants development. P. tomentosa and Arabidopsis were chosen as the research object in the paper. PtAUX1 complied with the role of auxin characteristics and influenced organ morphology through the regulation of auxin polar transport in the distribution of the plants. By using expression vector of P. tomentosa PtAUX1 constructed by our laboratory, the auxin influx import carrier PtAUX1 was transformed into Arabidopsis and Poplar. PtAUX1 transgenic plants were detected by PCR, and the phenotypes of transgenic plants and morphologic characters cultured in vivo were analyzed. The experiments showed that the over-expression of PtAUX1 influenced many organs of the morphological development. Transgenic plant of Poplar had vigorous growth and spire. Transgenic plant of Arabidopsis had grown in groveling, not altered root geotropism, trichome number increased on the stem, and abnormal floral organs. The results indicated that PtAUX1 promoted cells and tissues to absorb endogenesis IAA by auxin polar transport, and then participated in organ polarity establishment, and influenced morphology and development of the organs.