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Chinese Agricultural Science Bulletin ›› 2012, Vol. 28 ›› Issue (16): 165-170.doi: 10.11924/j.issn.1000-6850.2012-0525

Special Issue: 园艺

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Molecular Marker and Genetic Analysis of Cucumber Seed Length

  

  • Received:2012-02-23 Revised:2012-03-14 Online:2012-06-05 Published:2012-06-05

Abstract:

In order to become the correct policy with cucumber seed breeding plan and efficient variety breeding, it is necessary to study in detail the cucumber seed length. Selected five different seed length inbred lines and build hybrid combination according GriffingⅡ. The results showed that cucumber seed length was a quantitative trait. It took dominant effect as main character,and there were certain additive effects. The narrow-sense heritability and broad-sense heritability were 28.6777% and 10.8384%. On the basis of F2 segregating population derived from combinations of large seed D06157 and small seed D0603, a molecular marker linkage map was constructed with SSR marker, and QTL analysis was performed with composite interval mapping(CIM). The results showed that four linkage groups were constructed which containing 16 SSR loci, and the total length of the linkage group was 287.1 cM, the average distance between markers was 17.94 cM. Six QTLs was detected. Among them QTL5 and QTL6 had higher contribution rate (>5%) on seed length, the minimum distance were 2.1 cM, 4.3 cM, LOD value were 7.84, 9.69, and the contribution rate to mutation were 6.07%, 6.08%, the additive effects were 39.12%, 37.21%.

CLC Number: