Abstract: For the purpose of making better use of sweet potato native cultivars in Yunnan, we collected and evaluated 27 native sweet potato cultivars in Yunnan Province, and the 6 native sweet potatoes were had virus-free. The results showed some of native cultivar were of high yield, such as‘Puer Huangshu’(20.04 t/hm²),‘Qiubei Beipi’(15.60 t/hm²), some were of high quality, such as‘Yanhe Banlishu’and‘Huapin Zixin’. After removing virus by shoot apex culture, the yields of six native sweet potato were had virus-free,‘Yanhe Banlishu’,‘Jianshui Huishu’,‘Huaping Zixin’,‘Malong Zishu’,‘Kunming Zishu’,‘Jianshui Zishu’increased by 175.8%-200.1%, the yield of‘Jianshui Huishu’was 24.00 t/hm², increased by 200.1%. It is recommended to collect the native sweet potato and remove the virus by shoot apex culture. It will provide useful sweet potato cultivar for production.