Abstract: In order to study comprehensive effect of sowing dates, plant densities, nitrogenous fertilizer, phosphorus fertilizer, potassium on quality combination parameters of high grade hybrid rape. Experiment material is ‘Youyan 599’ and ‘Sanbei 98’, study on effects of cultivation factor on quality combination parameters of high grade hybrid rape were carried out through orthogonal rotational regression combination design in Brassica napus L. The results showed that, there were negative correlations between protein & fat content and sowing dates (-0.7493, -0.3846), positively correlated with nitrogenous fertilizer (0.8927*, 0.8977*), and potassium (0.5851, 0.7530). There were positively correlations between unsaturated fatty acid and plant densities (0.2086, 0.6696), nitrogenous fertilizer (0.7913, 0.6980), negative correlated with phosphorus fertilize (-0.6350, -0.4162). There were positively correlations between saturated fatty acid and sowing dates (0.4329, 0.7887), phosphorus fertilize (0.8928*, 0.3449). There were positively correlations between index of unsaturated fatty acid and plant densities (0.5447, 0.5721), nitrogenous fertilizer (0.9208*, 0.8863*), negative correlated with phosphorus fertilizer (-0.2442, -0.3468), potassium (-0.3562, -0.6703). There were negative correlations between ratio of unsaturated fatty acid and saturated fatty acid and sowing dates (-0.1264, -0.7246), phosphorus fertilizer (-0.8513, -0.7480), positively correlations with nitrogenous fertilizer (0.5380, 0.5406). There were positively correlations between 18C fatty acid content and plant densities (0.2387, 0.7053), nitrogenous fertilizer (0.7866, 0.7200), negative correlations with phosphorus fertilizer (-0.6438, -0.3948). There were negative correlations between total fatty acid content of erucic acid chain and sowing dates (-0.7400, -0.4480), nitrogenous fertilizer (-0.5999, -0.9558*), positively correlations with plant densities (0.8918*, 0.0923). There were positively correlations between ratio of unsaturated fatty acid and total fatty acid content of erucic acid chain and sowing dates (0.7014, 0.3873), nitrogenous fertilizer (0.6824, 0.9614**), potassium (0.0502, 0.0018). There were positively correlations between oleic acid & linoleic acid and plant densities (0.3146, 0.6348), nitrogenous fertilizer (0.7429, 0.5280), negative correlated with phosphorus fertilize (-0.6286, -0.4431). There were positively correlations and negative correlations among cultivation factor and quality combination parameters influence of each cultivation factor on quality combination parameters, positively correlations and negative correlations could be counteract each other. Expression of quality combination parameter for each treat was comprehensive influence of five cultivation factors on quality combination parameter. It was bigger for effect of five cultivation factors on ratio of unsaturated fatty acid and total fatty acid content of erucic acid chain of two varieties, it was smaller for effect of five cultivation factors on protein & fat content of two varieties, oil quality of high grade hybrid rape could improvement to a certain extent through adjusting cultivation measure, phosphorus fertilizer was a cultivation factor that produce negative effect on oil quality of high grade hybrid rape.