Abstract:

The OGP protein was important in the in vitro fertilization of pig. In order to produce the recombination OGP protein, the OGP secretory expression vector was constructed. The total RNA was extracted from porcine oviduct and coding sequence of porcine OGP was amplified using RT-PCR method. The OGP CDS was cloned into the pET22b vector and then identified by restriction enzyme analysis, PCR amplification and DNA sequencing. The results showed that the pET22b-OGP vector was constructed successfully, which provided advantage for further study of OGP function at the porcine in vitro fertilization.