The Standardization of the Collection and Treatment Methods for Swine Nasal Swab Sample

doi:10.11924/j.issn.1000-6850.2012-2096

Abstract

Abstract: The comparison and stability of detecting data were determined by the standard of materials and methods applied to collect mucosal samples. This study combined nasal cavity construction in pig and the specificity of the samples to investigate and standardize the collection and treatment methods of nasal swabs. The results showed that the total protein of 116 nasal swabs, collected by the methods standardized in this study, were detected, found that the total protein mean of all nasal swabs was (6.92±0.41) mg/mL and coefficient of variation was 32.58%. Besides, total protein content of the nasal swabs collected in five different times from 7 pigs were detected and the coefficient of variation of each pigs were below 10%, except one was 13.95%. The standard of the collection and treatment methods for nasal swabs made the detected results more stability and scientific.

References

[1] 杨伟平.口腔黏膜免疫研究进展[M].国外医学空腔医学分册,2001,28 (3):183-186.
[2] 葛忠源,熊东艳,张启勇.黏膜免疫的研究进展[J].青海畜牧兽医杂志, 2008,38(5):44-46.
[3] Bernstein J M, Reddy M S, Scannapieco F A, et al. The microbial ecology and immunology of the adenoid: implications for otitis media[J]. Ann. N. Y. Acad. Sci,1997,830:19-31.
[4] Mungan D, Misirligil Z, Gurbuz L. Comparison of the efficacy of subcutaneous and sublingual immunotherapy in mite-sensitive patients with rhinitis and asthma—a placebo controlled study[J].Ann. Allergy Asthma Immunol,1999,82:485-490.
[5] Varga E M, Nouri-Aria K, Till S J, et al. Immunomodulatory treatment strategies for allergic diseases[J].Curr. Drug Targets Inflamm Allergy, 2003,2:31-46.
[6] Gidaro G B, Marcucci F, Sensi L, et al. The safety of sublingual-swallow immunotherapy: an analysis of published studies[J].Clin Exp Allergy, 2005,35:565-571.
[7] Rienzo V D, Minelli M, Musarra A, et al. Post-marketing survey on the safety of sublingual immunotherapy in children below the age of 5 years [J].Clin Exp Allergy,2005,35:560-564.
[8] Baroody F M, Mucha S M, Detineo M, et al. Nasal challenge with allergen leads to maxillary sinus inflammation[J].J Allergy Clin Immunol, 2008,121:1126-1132.
[9] Hendley J O, Gwaltney Jr J M. Viral titers in nasal lining fluid compared to viral titers in nasal washes during experimental rhinovirus infection[J]. J Clin Virol,2004,30:326-328.
[10] Fabien X L, Robert E E. Novel nasal secretion collection method for the analysis of allergen specific antibodies and inflammatory biomarkers[J].J Immunol Methods,2010,356(1-2):6-17.
[11] Klimek L, Wolf H, Mewes T, et al. The effect of short-term immunotherapy with molecular standardized grass and rye allergens on eosinophil cationic protein and tryptase in nasal secretions[J].J. Allergy Clin.Immunol,1999,103:47-53.
[12] Heikkinen T, Shenoy M, Goldblum R M, et al. Quantification of cytokines and inflammatory mediators in samples of nasopharyngeal secretions with unknown dilution[J].Pediatr Res,1999,45:230-234.
[13] Niehaus M D, Gwaltney J M J, Hendley J O, et al. Lactoferrin and eosinophilic cationic protein in nasal secretions of patients with experimental rhinovirus colds, natural colds, and presumed acute communityacquired bacterial sinusitis[J].J Clin Microbiol,2000,38: 3100-3102.
[14] Millqvist E, Ternesten-Hasseus E, Stahl A, et al. Changes in levels of nerve growth factor in nasal secretions after capsaicin inhalation in patients with airway symptoms from scents and chemicals[J].Environ Health Perspect,2005,113:849-852.
[15] Ventura M, Toungoussova O, Foschino B M, et al. Validity and reproducibility of morphologic analysis of nasal secretions obtained using ultrasonic nebulization of hypertonic solution[J].Ann Allergy Asthma Immunol,2007,99,232-235.
[16] Watelet J B, Gevaert P, Holtappels G, et al. Collection of nasal secretions for immunological analysis[J].Eur.Arch. Otorhinolaryngol,2004,261(5): 242-246.
[17] Kristiansen B E. Collection of mucosal secretion by synthetic discs for quantitation of secretory IgA and bacteria[J].J Immunol Methods,1984,73 (2):251-257.
[18] Edward E W, Ann R F. A Simple and Reproducible Method for Collecting Nasal Secretions in Frail Elderly Adults, for Measurement of Virus-Specific IgA[J].The Journal of Infectious Diseases,1999,179(5): 1268-73.
[19] 内蒙古农牧学院,安徽农学院主编.家畜解剖学及组织胚胎学[M].北京:农业出版社,1981:182-183.
[20] McNeilly T N, Naylor S W, Mitchell M C, et al. Simple methods for measurement of bovine mucosal antibody responses in vivo[J].Vet Immunol Immunopathol,2007,118(1-2):160-167.
[21] Baraniuk J N, Kaliner M. Neuropathies and nasal secretion[J].Am J Physiol,1991,261:223-235.
[22] Greiff L, Akerlund A, Andersson M, et al. Day – night differences in mucosal plasma proteins in common cold[J].Acta Otolaryngol,1996,116: 85-90.