Advancement of Major Immunogenicity Proteins on Mycoplasma Hyopneumoniae

doi:10.11924/j.issn.1000-6850.2012-2419

Abstract

Abstract: Mycoplasma hyopneumoniae (Mhp) was the causative agent of Mycoplasma pneumoniae of Swine (MPS), and causes huge economic losses to the pig industry. Due to the lack of depth study for immunogenic and specific proteins of Mhp, the development of the pathogenic mechanism, serological detection methods and new recombinant subunit vaccine research about Mhp were restricted to some extent, so it affected the control of MPS. In this article, the development of some major specific immunogenicity proteins (including adhesion, membrane proteins and cytoplasmic protein) research of Mhp were discussed, so as to provide theoretical basis and new ideas for the further research on the pathogenesis of Mycoplasma hyopneumoniae, the establishment of specific diagnostic methods and promoting the development of Mhp genetic engineering vaccine, it would play an important part in correctly diagnosis, detecting, the immunization and prevention of MPS.

References

[1] Straw B E, Zimmerman J J, Allaire S D, et al. 猪病学[M].赵德明,张仲秋,沈建忠,等译.北京:中国农业大学出版社,2008:795-815.
[2] Zhang Q J, Young T F, Ross R F, et al. Indentification and characterization of a Mycoplasma hyopneumoniae adhesion[J]. Infection andImmunity,1995,63(3):1013-1019.
[3] 李桂兰,刘志宗,张映.猪肺炎支原体致病机理研究进展[J].南方养猪,2007(222):4-7.
[4] Tsungda H, Sergey A, Chris M F. Cloning and functional analysis of the P97 swine cilium adhesion gene of Mycoplasma hyopneumoniae[J].Journal of Bacteriology,1997,179(4):1317-1323.
[5] 刘茂军,邵国青,张映.猪肺炎支原体不同毒力株黏附因子基因序列分析[J].江苏农学报,2010,26(3):522-525.
[6] Thacker E L, Thacker B J, Kuhn M, et al. Evaluation of local and systemic immune responses induced by intramuscular injection of a Mycoplasma hyopneumoniae bacterin to pigs[J].American journal of veterinary research,2000,61(11):1384-1389.
[7] Feng Z X, Shao G Q, Liu M G, et al. Development and validation of a SIgA-ELISA for the detection of Mycoplasma hyopneumoniae infection[J].Veterinary Microbiology,2010,143(2010):410-416.
[8] 苏锐,邵国青,张映.猪肺炎支原体P97基因原核表达产物单抗的制备[J].中国人兽共患病杂志,2005,21(3):202-205.
[9] Faust R O, Maximilien A, Nedzad M, et al. Potential use of a recombinant replication-defective adenovirus vector carrying the C-terminal portion of the P97 adhesin protein as a vaccine against Mycoplasma hyopneumoniae in swine[J].Vaccine,2010(28): 4802-4809.
[10] 陈超,李媛,郭丹,等.猪肺炎支原体p97C末端基因重组腺病毒的构建及其免疫效果[J].微生物学报,2009,49(4):0465-0470.
[11] Tracey A B, Katrin D, Manfred R, et al. P159 is a proteolytically processed, surface adhesin of Mycoplasma hyopneumoniae: defined domains of P159 bind heparin and promote adherence to eukaryote cells[J].Molecular Microbiology,2006,60(3):669-686.
[12] 曹培丽,李媛,陈超,等.猪肺炎支原体 P52蛋白的原核表达及抗血清制备[J].中国预防兽医学报,2009,31(7):572-576.
[13] Tsungda H, Sergey A F. Chris M. Cloning and functional analysis of the P97 swine cilium adhesion gene of Mycoplasma hyopneumoniae [J].Journal of Bacteriology,1997,179(4):1317-1323.
[14] Adams C, Pitzer J, Minion F C. In vivo expression analysis of the P97 and P102 paralog families of Mycoplasma hyopneumoniae[J]. Infection and Immunity,2005,73:7784-7787.
[15] Chen J R, Lin J H, Weng C N, et al. Identification of a novel adhesion-like glycoprotein from Mycoplasma hyopneumoniae[J]. Veterinary Microbiology,1998,62:97-110.
[16] Jody W, Cheryl J, Stuart J C, et al. Mhp493 (P216) is a proteolytically processed, cilium and heparin binding protein of Mycoplasma hyopneumoniae[J].Molecular Microbiology,2009,71 (3):566-582.
[17] Lisa M S, Ania T D, Cheryl J, et al. A Processed Multidomain Mycoplasma hyopneumoniae adhesin binds Fibronectin, Plasminogen, and Swine Respiratory Cilia[J].The American Society for Biochemistry and Molecular Biology,2010,285:33971-33978.
[18] Ania T D, Cheryl J, Minion F C, et al. Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine ciliammi[J].Molecular Microbiology,2010,78(2):444-458.
[19] Chris M F, Elliot J, Melissa L, et al. The Genome Sequence of Mycoplasma hyopneumoniae Strain 232, the Agent of Swine Mycoplasmosis[J].Journal of Bacteriology,2004,186(21):7123-7133.
[20] Futo S, Seto Y, Okada M, et al. Recombinant 46-Kilodalton Surface Antigen (P46) of Mycoplasma hyopneumoniae Expressed in Escherichia coli Can Be Used for Early Specific Diagnosis of Mycoplasmal Pneumonia of Swine by Enzyme-Linked Immunosorbent Assay[J].Journal Clinical Microbiology,1995,33(3): 680-683.
[21] Cheikh S B K, Shareck F, Dea S. Monoclonal Antibodies to Escherichia coli-Expressed P46 and P65 Membranous Proteins for Specific Immunodetection of Mycoplasma hyopneumoniae in Lungs of Infected Pigs[J].American Society for Microbiology,2003, 10(3):459-468.
[22] Satoshi F, Yasuhiro S, Munenori O, et al. Recombinant 46-Kilodalton Surface Antigen (P46) of Mycoplasma hyopneumoniae Expressed in Escherichia coli Can Be Used for Early Specific Diagnosis of Mycoplasmal Pneumonia of Swine by Enzyme-Linked Immunosorbent Assay[J].Journal of clinical microbiology,1995,33(3):680-683.
[23] 刘志宗,葛亚明,宁红梅,等.猪肺炎支原体 P46基因特异性噬菌体随机肽库的构建[J].畜牧与兽医,2009,41(10):51-54.
[24] Jochen M, Martin S, Gerald F G. Identification and immunological characterization of conserved Mycoplasma hyopneumoniae lipoproteins Mhp378 and Mhp651[J].Veterinary Microbiology,2006, 116:85-95.
[25] Peter K F, Steven P D, Graeme J E, et al. Molecular Characterization of a Ribonucleotide Reductase (nrdF) Gene Fragment of Mycoplasma hyopneumoniae and Assessment of the Recombinant Product as an Experimental Vaccine for Enzootic Pneumonia[J].Infecion and Immunity,1996,64(3):1060-1064.
[26] Meens A J, Bolotin V, Frank R, et al. Characterization of a highly immunogenic Mycoplasma hyopneumoniae lipoprotein Mhp366 identified by peptide-spot array[J].Veterinary Microbiology,2010, 142(2010):293-302.
[27] Upton C, Buckley J T. A new family of lipolytic enzymes[J].Trends Biochem Sci,1995,20(5):178-179.
[28] Schmidt J A, Browning J F, Markham P F, et al. Mycoplasma hyopneumoniae p65 Surface Lipoprotein Is a Lipolytic Enzyme with a Preference for Shorter-Chain Fatty Acids[J].Journal of Bacteriology,2004,186(17):5790-5798.
[29] Chou S Y, Chung T L, Chen R J, et al. Molecular cloning and analysis of a HSP (heat shock protein)-like 42kDa antigen gene of Mycoplasma hyopneumoniae[J].Biochemistry and Molecular Biolory International,1997,41(4):821-831.
[30] Chen Y L, Wang S N, Yang W J, et al. Expression and Immunogenicity of Mycoplasma hyopneumoniae Heat Shock Protein Antigen P42 by DNA Vaccination[J].Infection and Immunity,2003,71(3):1155-1160.
[31] 王亮,乔祖健,李媛.猪肺炎霉形体 Dnak-P97融合基因的表达及其产物的生物学活性[J].中国兽医科学,2008,38(2):123-127.
[32] 李媛,张建华,王亮.猪肺炎支原体中国分离株热休克蛋白 Hsp70 (Dnak)的全基因克隆及 C末端基因的表达与免疫活性分析[J].中国预防兽医学报,2008,30(3):169-173.
[33] 乔祖健,王亮,李媛,等.猪肺炎霉形体热休克蛋白 Hsp70单克隆抗体的制备及鉴定[J].中国兽医科学,2008,38(8):701-706.
[34] 刘茂军,丁志勇,杜海霞,等.猪肺炎支原体 Dnak 基因的表达及ELISA方法的建立[J].金陵科技学院学报,2011,27(14):1-7.
[35] 祝永琴,冯志新,刘茂军,等.猪肺炎支原体 P36蛋白间接 ELISA检测方法的建立[J].江苏农业学报,2010,26(5):987-992.
[36] Brooks E, Faulds D. The Mycoplasma hyopneumoniae 74.5 kD antigen elicits neutralizing antibodies and shares sequence similarity with heat-shock proteins[J].Vaccine,1989,89:265-269.
[37] Feld N C, Qvist P, Ahrens P, et al. A monoclonal blocking ELISA detecting serum antibodies to Mycoplasma hyopneumoniae[J]. Veterinary Microbiology,1992,30:35–46.
[38] Caron N J, Sawyer B, Benabdel M, et al. Species-Specific Monoclonal Antibodies to Escherichia coli-Expressed p36 Cytosolic Protein of Mycoplasma hyopneumoniae[J].Clinical and Diagnostic Laboratory Immunology,2000,7(4):528-535.
[39] 祝永琴,刘茂军,冯志新,等.猪肺炎支原体 P36基因在毕赤酵母中的表达[J].江苏农业学报,2011,27(2):307-312.
[40] 姚睿智,何启盖,周锐,等.猪肺炎支原体乳酸脱氢酶基因的克隆、表达、纯化及其间接 ELISA检测方法的建立[J].中国兽医学报,2007, 27(4):511-515.
[41] Yang W J, Lai J F, Peng K C, et al. Epitope mapping of Mycoplasma hyopneumoniae using phage displayed peptide libraries and the immune responses of the selected phagotopes[J]. Journal of Immunological Methods,2005,304(2005):15-29.