Abstract:

In order to explore the resource diversity and microbial inhibition activity of endophytic fungi from endangered medicinal plant Ammopiptanthus Mongolicus Cheng f., the endophytic fungi were isolated from root, stem and leaf of the host by tissue inoculation culture and five pathogenic fungi and four bacteria strains used as indicators to test microbial inhibition activity by agar plate antagonistic action and modified agar gel diffusion methods. The results indicated that forty-one endophytic fungi strains were isolated from the host, most of them coming from root, then leaf and stem the least; The isolated strains attribute to nineteen genera, six families, and five orders based on morphological characters; for the isolated strains thirty-one strains were found to have some microbial inhibition activities against 2 or more indicators, making up 75.6% of the total isolated strains , fifteen isolated strains had antibacterial activity against 1 or more indicating bacteria to some degree, amounting to 36.6% of the total isolates, among which five endophytic fungi strains isolated showed evident inhibition activity against 4 indicating pathogenic fungal strains respectively, which was 12.2% of the isolated strains, eight strains isolated exhibited evident inhibition activity against 1 indicating bacteria, respectively, which was 19.5% of the total isolates, there were four strains showed evident antifungal and antibacterial activity with broad spectrum, belonging to Penicillium sp., Ozonium sp., and Ectostroma sp.. The endangered medicinal plant Ammopiptanthus Mongolicus Cheng f. showed abundant endophytic fungal resource, and its development and application is of ecological and economic significance.