Abstract: In order to study the similarities and differences between medium vocational graduates and higher vocational graduates in vocational college, further differentiated instruction could be applied. In this paper, questionnaires were used to study about the two sorts of students at the end of first-year course, the learning purpose and major recognition, learning confidence and course satisfaction, master the knowledge and learning about professional courses, life satisfaction and career development were contained. The results showed that both of them had well performance in learning objectives, learning interest and confidence, agreed with the professional qualification examination, thought the vocational school diploma couldn't meet their demand. Instead, time and initiative in expanding professional knowledge, professional level and career development were with poor performance. For the medium vocational graduates, teachers should focus more on their ability to expand professional knowledge, take various measures to mobilize the specialty identification and interest, and improve their confidence and patience in learning.