Abstract: In order to detect genotype of extend spectrum β-lactamase (ESBLs) in E. coli isolates from different animal, and explore the influence of ESBLs to E. coli resistance, to instruct to prevent and cure Colibacillosis in veterinary clinic effectively. We detected antimicrobial susceptibility and ESBLs for 102 E. coli strains by NCCLS standard method and Double-disk Synergy Test, and designed and synthetized four pair of primers, studied ESBLs gene type with amplified and analyzed by PCR. The results showed that the ESBLs producing bacteria amplified by PCR with TEM-type and CTX-M-type down-object while SHV-type and OVA-type down-object did not. The detection rate of the ESBLs producing E. coli strains from chickens and pigs is 81% and 6% respectively. CTX-M-type and TEM-type were epidemic genotype of ESBLs in Shandong Laixi Region, and the ESBLs producing E. coli strains from chickens were widespread. We should reinforce monitoring and studying about the ESBLs producing drug-resistant bacteria in order to prevent and cure Colibacillosis in veterinary clinic effectively.