Abstract:

The aim was to screen the suitable DNA extraction method for Ganoderma pseudoferreum of rubber tree and evaluate the effect. The purity and output of Ganoderma pseudoferreum DNA extracted by three improved method including CTAB, benzyl chloride and SDS were compared via ultraviolet absorption spectrum and agarose gel electrophoresis. The best method was used to extract the DNA of five culture time (4, 6, 8, 10, 12 d) and detected by ITS-PCR. The purity and output via improved CTAB showed the best results, OD260/OD280 was 1.82-1.84 and DNA concentration was 280 μg/mL. Cultivating for 4-8 d was the suitable time for DNA extraction and PCR detection. The improved CTAB method was suitable for Ganoderma pseudoferreum DNA extraction.